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. 2017 Aug 23;8:1469. doi: 10.3389/fpls.2017.01469

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Copyright © 2017 Sattar, Iqbal, Tahir, Shahid, Khurshid, Al-Khateeb and Al-Khateeb.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The CRISPR/Cas9-based resistance model in date palm depicting the recognition and disruption of the pathogen genetic material in three steps: acquisition, expression, and interference). During acquisition the invading DNA is integrated and duplicated into the CRISPR-locus at the leader side. The expression step involves the active transcription and expression of pre-CRISPR RNA (Pre-crRNA), which is further processed into mature crRNAs specifically with the help of different Cas proteins. During the third step of interference, any complementary target region of the foreign genetic material is recognized and cleaved as guided by crRNA and Cas9 protein.