FIGURE 3.

Schematic presentation of multiplex genome editing strategy in date palm. A preferred assemblage of multiplex cassette is shown for different sgRNAs to target different genes simultaneously. The spacers and sgRNA scaffold can be inserted between adjacent tRNAs followed by a NOS terminator at the end. The second cassette can be expressed from Pol-III promotor and NOS terminator sequences sharing the same binary vector with sgRNA cassette. The whole multiplex cassette can then be transcribed and expressed separately in the date palm genome to carry out genome editing.