Table 2.

Summary of the approaches to culture naïve BM-MSCs in the serum free culture with growth factor supplements.

Marker used	Approach to culture naïve BM-MSC	Fate of naïve MSCs	References
Stro-1/ CD45-	Stro-1/CD45- BM-MSCs cells were immunomagnetically sorted and cultured in vitro using serum free alpha-MEM media formulation (BSA, insulin, transferrin and low-density lipoprotein) with various growth factors and or L-ascorbate. The Stro-1+ cells also expressed CD271, PDGF-R and EGF-R.	CFU-F was increased in the presence of L-ascorbate, PDGF and EGF. The maintenance of Stro-1 and or CD271 expression during in vitro culture was not studied.	Gronthos and Simmons, 1995
Ficoll separated human BM cells	BM mononuclear cells were cultured and maintained in a serum-free media containing IMDM supplemented with 1% BSA, 5 ug/ml of human insulin, 100 ug/ml of human transferrin, 10 ug/ml of low-density lipoprotein, 10∧-4 M beta-mercaptoethanol and growth factors (SCF, and IL-3).	CD123+/CD45- mesenchymal progenitor cells with osteogenic differentiation ability could be expanded for 3weeks.	Baksh et al., 2003
Ficoll separated human BM cells.	Stirred suspension culture in Stem Span media, (StemCell Technologies, Vancouver, BC) with SCF and IL-3.	Concomitant growth of both CD45+ and CD45- cells was observed. CD123+/CD45- cells were maintained for 3 weeks and showed 3-fold increase in number.	Baksh et al., 2005
CD271+/ CD56+, and CD271+/ CD56-	CD271+/CD56+ or CD271+/CD56- cells were grown in serum free media with FGF-2 supplement. Duration of in vitro culture is not mentioned.	CD271+ expression was lost, whereas the expression of CD166 and CD318 were increased.	Battula et al., 2009
CD133, CD271, CD45	Flow cytometry sorted population of CD133+/CD271+/CD45- BM-MSCs were cultured in vitro in serum rich media.	Loss of CD133 and CD271 marker expression. Also, marked reduction in CFU-F formation as compared to freshly obtained CD133+/CD271+/CD45- cells.	Bakondi et al., 2009
CD146, CD271	CD146+/CD271+ BM-MSCs were maintained in serum rich media.	CD271 expression in BM-MSCs was decreased during the in vitro culture system using serum rich media.	Tormin et al., 2011
CD133+CD271+ BM cells	CD133+/CD271+ cells that contain both HSCs and MSCs were cultured in serum free StemSpan media with growth factors SCF, TPO, and Flt3. IL-3 was not added to avoid the expansion of osteogenic progenitor as observed by Baksh et al. (2003).	92% of CD271+ BM-MSCs maintained stemness for 2 weeks, and showed 2-fold expansion.	Das et al., 2013
CD34+/ CD45+ and CD271+/CD45-	CD271+ BM-MSCs were co-cultured with human umbilical cord blood CD34+ cells in serum free media with SCF, TPO, and Flt3. In vitro culture was done for a week.	CD271+ cell surface marker expression was preserved for 1 week.	Li et al., 2014