Figure 2.

Localization of Src kinase and Dyn2 during HSV-1 neuronal infection and Src kinase inhibition. Cortical primary neurons were left untreated (HSV-1) or treated (HSV-1 + PP2) with 20 μM PP2 for 12 h, and then, cells were either mock-infected (MOCK) or HSV-1 infected (moi = 10). After 4, 8, and 18 h post-infection (hpi) cells were fixed, permeabilized, and incubated with the rabbit polyclonal antibodies to either p-Src (Upper) or p-Dyn2 (Lower) and with the mouse monoclonal antibody to ICP8 (both panels). Next, cells were incubated Alexa Fluor 488–conjugated donkey anti-rabbit IgG (green channels) and Alexa Fluor 594–conjugated donkey anti-mouse IgG (red channels). DAPI stain was used to visualize nuclei. Images were acquired by fluorescence microscopy Merging green and red channels generated a third image show in each row. Scale bar, 5μm. For visualization of neuronal morphology see also Figure S1.