Table 3. Molecular evolutionary analyses between three Cherax crayfish (C. quadricarinatus, C. destructor and C. cainii) at amino acid/codon level.

	CAc	CAg	CAb	NKA	HAT-116k	NKCC	NBC	NHE3	NCX1	AK	SERCA	CRT
Total sites/positions in analysis	271	277	257	1,038	831	848	1,145	921	846	357	1,002	402
Identical sites in all three species	262	273	252	1,035	819	811	1,121	830	831	347	979	395
Nonsynonymous sites	9	4	5	3	12	37	24	91	15	10	23	7
Synonymous substitutions at codon	54	57	18	71	85	506	107	143	91	26	55	39
Divergent sites in all three species	0	0	0	0	0	1	1	9	1	1	1	0
Unique differences in Redclaw	3	0	0	1	6	11	7	29	7	1	6	1
Unique differences in Yabby	3	2	3	2	1	14	7	20	2	3	9	3
Unique differences in Marron	3	2	2	0	5	11	9	33	5	5	7	3
Equal evolutionary rate between Redclaw & Yabby?	Yes (1.00)a	Yes (0.16)	Yes (0.08)	Yes (0.56)	Yes (0.06)	Yes (0.55)	Yes (0.8)	Yes (0.20)	Yes (0.10)	Yes (0.32)	Yes (0.44)	Yes (0.32)
Equal evolutionary rate between Redclaw &Marron?	Yes (1.0)	Yes (0.16)	Yes (0.16)	Yes (0.32)	Yes (0.76)	Yes (1)	Yes (0.8)	Yes (0.61)	Yes (0.56)	Yes (0.10)	Yes (0.78)	Yes (0.32)
Equal evolutionary rate between Yabby & Marron?	Yes (1.0)	Yes (1.00)	Yes (0.65)	Yes (0.16)	Yes (0.10)	Yes (0.55)	Yes (0.6)	Yes (0.07)	Yes (0.26)	Yes (0.48)	Yes (0.62)	Yes (1.0)
Average evolutionary divergence	0.022	0.010	0.013	0.002	0.010	0.03	0.05	0.072	0.012	0.02	0.016	0.012

Notes.

CAc

Cytoplasmic carbonic anhydrase

CAg

GPI-linked carbonic anhydrase

CAb

Beta carbonic anhydrase

NKA

Na⁺/K⁺-ATPase alpha subunit

HAT-116k

Vacuolar type H⁺-ATPase 116 kda

NKCC

Na⁺/K⁺/2Cl⁻ cotransporter

NBC

Na⁺/HCO${}_3^{-}$cotransporter

NHE3

Na⁺/H⁺ exchanger 3

NCX1

Na+/Ca⁺² exchanger 1

AK

Arginine kinase

SERCA

Sarco/endoplasmic reticulum Ca⁺²-ATPase

CRT

Calreticulin

^aValues in brackets are p-values used to reject the null hypothesis of equal evolutionary rate.