Table 1.
Summary of technical aspects and major contributions of publications to date using optogenetic and chemogenetic techniques in nonhuman primates.
| Publication | Species | Viral Vector
|
Transduction Target | ||
|---|---|---|---|---|---|
| Virus | Promoter | Transgene | |||
| Optogenetic Studies | |||||
|
| |||||
| Han et. al 2009 | Macaca mulatta | Lenti | CaMKIIα | ChR2 | Cortex (PMC and FEF) |
| Diester et. al 2011 | Macaca mulatta | Lenti, AAV5 | hThy1, hSyn | ChR2, eNpHR2, SFO | Cortex (SS, M1) |
| Han et. al 2011 | Macaca mulatta | Lenti | CaMKIIα | ArchT | Cortex (Par., V1) |
| Cavanaugh et. al 2012 | Macaca mulatta | AAV8 | CAG | ArchT | SC |
| Galvan et. al 2012 | Macaca mulatta | Lenti | Ef1α | ChR2 | Thalamus (VL) and putamen |
| Gerits et. al 2012 | Macaca mulatta | AAV5 | CAG | ChR2 | Cortex (Ventral PMC and FEF) |
| Jazayeri et. al 2012 | Macaca mulatta | AAV1 | hSyn | ChR2 | V1 |
| Tamura et. al 2012 | Macaca mulatta & fuscata | Lenti | CMV | ChR2 | Thalamus (MD, VA, VL) |
| Ohayon et. al 2013 | Macaca (*) | AAV5 | hSyn, CAG, CaMKII | ChR2, ArchT, eNpHR3.0 | Cortex (FEF) |
| Ozden et. al 2013 | Macaca mulatta | AAV5 | CaMKIIα | C1V1 | Cortex (SS, PMC) |
| Ruiz et. al 2013 | Macaca mulatta | Lenti, AAV5 | CaMKIIα | ChR2, ArchT, C1V1 | V1 |
| Dai et. al 2014 | Macaca mulatta | AAV5 | CaMKIIα | C1V1 | Cortex (parietal) |
| Huang et. al 2014 | Tupaia belangeri | AAV2/9 | CaMKIIα, hSyn | ChR2 | V1 |
| May et. al 2014 | Macaca mulatta | AAV5 | CaMKIIα | C1V1 | SS |
| Afraz et. al 2015 | Macaca mulatta | AAV8 | CAG | ArchT | STS |
| Inoue et. al 2015 | Macaca mulatta | AAV2 | CMV | ChR2 | Cortex (FEF) |
| Lu et. al 2015 | Macaca (*) | AAV5 | CaMKIIα | C1V1 | Cortex (PMv, M1) |
| Nassi et. al 2015 | Macaca mulatta | Lenti, AAV5 | CaMKIIα | C1V1, eArch3.0 | V1 |
| Acker et. al 2016 | Macaca mulatta | AAV8 | hSyn | Jaws | Cortex (FEF) |
| Galvan et. al 2016 | Macaca mulatta | AAV5 | CaMKIIα | ChR2 | Cortex (PMC, M1) |
| Klein et. al 2016 | Macaca mulatta & fascicularis | AAV5 | CaMKIIα | ChR2 | LGN |
| MacDougall et. al 2016 | Callithrix jacchus | AAV5 | hSyn | ChR2 | Cortex |
| Stauffer et. al 2016 | Macaca mulatta | AAV5/9 | TH, Ef1α | Cre, ChR2 | Midbrain DA neurons |
| Yazdan-Shahmorad et. al 2016 | Macaca mulatta | AAV5 | CaMKIIα | C1V1 | Cotex (M1, SS) |
|
| |||||
| Chemogenetic Studies | |||||
|
| |||||
| Eldridge et. al 2016 | Macaca mulatta | Lenti | hSyn | hM4Di | Cortex (OFC) |
| Grayson et. al 2016 | Macaca mulatta | AAV5 | hSyn | hM4Di | Amygdala |
| Nagai et. al 2016 | Macaca mulatta & fascicularis | Lenti, AAV2 | CMV, hSyn | hM4Di | Caudate |
Abbreviations: AAV, adeno-associated virus; ArchT, archaerhodopsin from Halorubrum strain TP009; C1V1, hybrid of channelrhodopsin 1 and volvox carteri 1; C. Macaques, cynomolgus macaques; CaMKIIα, calcium and calmodulin-dependent protein kinase II α; CAG, cytomegalovirus early enhancer/chicken beta actin promoter; ChR2; channelrhodopsin-2; CMV, cytomegalovirus; DA, dopamine; DREADDs, designer receptor exclusively activated by designer drugs; eArch3.0, enhanced archaerhodopsin 3; Ef1α, elongation factor 1α; eNpHR, enhanced natronomonas pharaonic halorhodopsin; FEF, Frontal eye field; hM4Di, human muscarinic receptor 4 DREADD; hSyn, human synapsin; hThy1, human thymocyte-1; Lenti, lentivirus; LGN, lateral geniculate nucleus of thalamus; M1, primary motor cortex; MD, mediodorsal thalamus; OFC, orbito-frontal cortex; Par., parietal cortex; PMC, premotor cortex; PMv, ventral premotor cortex; R. Macaques, rhesus macaques; SC, superior colliculus; SFO, step-function opsin; SMA, supplementary motor area; SS, somatosensory cortex; STS, superior temporal sulcus; TH, tyrosine hydroxylase; V1, primary visual cortex; VA, ventroanterior thalamus; VL, ventrolateral thalamus.
species of macaca not specified