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. Author manuscript; available in PMC: 2018 May 19.
Published in final edited form as: Science. 2017 May 19;356(6339):717–721. doi: 10.1126/science.aal3096

Figure 1. Identification of kmg as an early differentiation gene.

Figure 1

(A) Male germ line stem cell lineage of Drosophila melanogaster.

(B) Number of EdU and pH3 positive cysts per testis and relative expression of Bam and Rbp4-YFP proteins in testes from hs-bam; bam−/− flies after transient induction of Bam by heat shock. Error bars: standard deviation (n > 15 testes).

(C) Comparison of transcript levels based on microarray in testes from no heat shock vs. 24 hour post heat shock (PHS) hs-bam; bam−/− flies (n = 2) (Red dots) Transcripts significantly upregulated (fold > 2, p < 0.05) at 24hr.

(D–E″) Immunofluorescence images of wild-type testis stained for (D) Kmg (green) and Vasa (red). (E- E″) high magnification of box in (D). (E″, merge) Kmg (green), DAPI (red). Dotted outline: spermatocyte nuclei.

Scale bars: 100μm in D; 10μm in E- E″.