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. Author manuscript; available in PMC: 2018 May 19.
Published in final edited form as: Science. 2017 May 19;356(6339):717–721. doi: 10.1126/science.aal3096

Figure 3. Kmg recruits dMi-2 to actively transcribed genes in spermatocytes.

Figure 3

(A) Phase-contrast image of a dMi-2 KD testis.

(B-B‴) Immunofluorescence images of (B) GFP visualized by native fluorescence, (B′) dMi-2, (B″) DAPI, (B‴, merge) DAPI (red), dMi-2 (green) in testis bearing germ line clones homozygous mutant for kmg (dotted line), marked by absence of GFP.

(C) Western blot of dMi-2, Kmg, Lysine Specific Demethylase 1 (Lsd1), and Actin after immunoprecipitation of (left) Kmg from wild-type and kmg KD or (right) GFP from dMi-2-GFP and wild-type testis lysates. Input: (Kmg IP) 15% or (dMi-2-GFP IP) 7.5% of lysate, FT: flow through after immunoprecipitation (equal proportion as input), IP: complete eluate after immunoprecipitation. Note: two bands in input lane from dMi-2-GFP testes, which express both dMi-2-GFP and untagged dMi-2.

(D) Box plot of expression of 440 de-repressed somatic transcripts in kmg KD, dMi-2 sibling control (no Gal4 driver) and dMi-2 KD testes. (whiskers) the most extreme data points excluding outliers.

(E) Genome browser screenshot showing ChIP-Seq results for Kmg and dMi-2. Y-axes: normalized read counts based on total one million mapped reads per sample. Y-axes for RNA-Seq reads are in log scale.

Scale bars: 100μm in A; 50μm in B-B‴.