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. Author manuscript; available in PMC: 2018 May 19.
Published in final edited form as: Science. 2017 May 19;356(6339):717–721. doi: 10.1126/science.aal3096

Figure 4. Kmg and dMi-2 prevent misexpression from cryptic promoters.

Figure 4

(A and C) Genome browser screenshots of RNA-Seq reads mapped to Crick (−) strand (pastel red) or mapped without strand information (gray) (embryo data from modENCODE) (27). Pastel blue lines: a single read that skipped an intron and mapped to two adjacent exons. Red arrows: cryptic promoter sites.

(B and D) Aly-HA ChIP and input read density in wild-type (with and without Aly-HA transgene) and kmg KD testes for the same regions. Red arrowheads: peak of enrichment by ChIP for Aly coinciding with potential cryptic promoter sites.

(E) Median RNA expression profile of 143 genes expressed in wild-type heads but not in wild-type testes and misexpressed in kmg KD testes, plotted as a metagene excluding introns. TSS: Transcription start site. TES: Transcription end site.

(F and G) Median profile of (F) RNA-Seq and (G) Aly ChIP (solid lines) and corresponding input (dotted lines) signals centered at cryptic promoters.

(H) Position weight matrix representation of sequence motif most significantly enriched in a collection of 181 cryptic promoters (±150bp around the TSS) with peaks of Aly binding in kmg KD testes.