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. 2017 Aug 13;2017:4602715. doi: 10.1155/2017/4602715

Figure 5.

Figure 5

SIRT1 regulated mitochondrial-related proteins expression through PGC-1α deacetylation. (a) Nonfunctional PGC-1α acetylation (about 105 kDa, indicated by the red box) was upregulated in the HG and SIRT1KD groups; meanwhile, SIRT1 activation in HG + RES cells promoted functional PGC-1α deacetylation. (b) HG and SIRT1KD decreased the mRNA expression of mitochondrion-related genes such as NRF-1, NRF-2, ERR-α, and TFAM (∗p < 0.05). SIRT1 activation by resveratrol upregulated the expression of NRF-1, NRF-2, ERR-α, and TFAM mRNA expression in HG + RES cells but not in SIRT1KD + HG + R cells (#p < 0.05, &p < 0.05). (c) Protein expression level changes of NRF-1, NRF-2, ERR-α, and TFAM showed the similar tendency with mRNA. ∗p < 0.05 versus Con; #p < 0.05 versus DCM; &p < 0.05 versus DCM + RES. NRF-1: nuclear respiratory factor 1; NRF-2: nuclear respiratory factor 2; ERR-α: estrogen-related receptor-α; TFAM: mitochondrial transcription factor A.