Skip to main content
. Author manuscript; available in PMC: 2017 Aug 28.
Published in final edited form as: Free Radic Biol Med. 2015 May 22;86:156–165. doi: 10.1016/j.freeradbiomed.2015.05.010

Fig. 2.

Fig. 2

CDKL5 fibroblasts show increased oxidative stress markers, i.e., 4-hydroxy-2-nonenal–protein adducts, nitrotyrosine–protein adducts, and overexpression of iNOS. (A) Representative Western blots for HNE–PA (left) and NT–PA (right) in healthy control (n=4) and CDKL5 (n=4) fibroblasts are shown. HNE–PA were present between 50 and 250 kDa, and bands reactive for NT–PA were observed in the range of 50 to 100 kDa. At the bottom is presented the Western blot quantification. (B) Immunofluorescence for HNE–PA (red) and NT–PA (green) in control and CDKL5 fibroblasts. Original magnification, ×630. Scale bar, 50 μm, valid for all images. (C) Left, a representative Western blot of iNOS protein levels in healthy control (n=4) and CDKL5 (n=4) fibroblasts. Right, quantification of the iNOS Western blot. Values are reported as arbitrary units. Data are means±SD of at least three independent experiments. *P<0.05.