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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Antiviral Res. 2017 Feb 2;141:62–72. doi: 10.1016/j.antiviral.2017.01.021

Fig. 4. B cells are increased in lungs of AuNP-M2e+sCpG vaccinated mice, and respond rapidly to in vitro restimulation with AuNP-M2e.

Fig. 4

B cell frequencies and CD86 expression was determined by flow cytometry. Hematopoietic cells were identified as CD45+ cells, and B cells as CD45+CD19+B220+CD3–cells. Study included two groups (n=5 per group, vaccinated and naïve). (A) Average frequency and standard deviation of B cells in the lungs, spleens and bone marrow (BM) of naïve vs. AuNP-M2e+sCpG vaccinated donor mice, regardless of their in vitro re-stimulation. (B) CD86 expression was determined on B cells isolated from naïve or AuNP-M2e+sCpG vaccinated donor lungs after 96 hours of media control or AuNP-M2e restimulation in vitro.