Figure 7.

PKM2 silencing in NP cells does not control hypoxic expression of HIF-1 targets. A) Targeting of the Pkm locus in human NP cells by 2 independent lentivirally delivered shRNA sequences resulted in decreased Pkm mRNA expression (n = 5). B–D) Western blot (B) and corresponding densitometric analysis (C, D) shows that both PKM1 and -2 levels are suppressed by either shRNA in NX (C) and HX (D) (n = 5). E) mRNA expression of HIF-1 target genes after PKM knockdown in NP cells (n = 5). Quantitative RT-PCR assays were performed with 2 technical replicates per experiment. Data are means ± sem. *P < 0.05.