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Blockage of activation of MAPKs by water-soluble p38 inhibitor 16 h after CLP, determined with techniques similar to those described in Fig. 2. A, C, E, G) At 16 h after CLP, CMs demonstrated phosphorylation of MAPKs p38 (A), ERK1/2 (C), and p-JNK (E) and AKT (G). Green: MAPK; blue: DAPI. B, D, F, H) Pre-CLP administration of the p38 inhibitor blocked activation in CMs of all 3 MAPKs (p38, B; ERK1/2, D; and p-JNK, F) and AKT (H). (n = 4 separate samples in each group).
