Figure 7.
ChQ activates TAS2Rs in cultured human myometrial cells. A, B) Efficiency of shRNA knockdown targeting human TAS2R14 (A) and TAS2R10 (B) as assessed by qPCR. C, D) [Ca2+]i rise induced by 10 mM ChQ is significantly suppressed by shRNA knockdown targeting human TAS2R14 (C), but not TAS2R10 (P = 0.75) (D). E, F) shRNA knockdown targeting human TAS2R14 (E), but not TAS2R10 (F), inhibits reversal by 1 mM ChQ of 600 nM OT-induced Ca2+ rise. Data are means ± sem; n = 30–73 cells. *P < 0.05, **P < 0.01; scrambled vs. shRNAi, unpaired Student’s t test.