Figure 3.

TAF9 recruitment correlates with HDAC1 deacetylase activity on human and mouse PU.1 promoter. A) K562 cells were treated with or without TSA for 8 h, and ChIP assay was performed with the indicated Abs. The resulting precipitated DNA was analyzed by using real-time PCR. B) ChIP assays with indicated Abs were performed in MEL cells that were treated with TSA or depsipeptide for 0 and 8 h. The resulting precipitated DNA was analyzed by using real-time PCR. C) MEL cells were induced with DMSO for 0 and 3 d. The recruitment of the TFIID complex on mouse PU.1 promoter was measured by ChIP assays with indicated Abs. The resulting precipitated DNA was analyzed by using real-time PCR. Depsi, depsipeptide; NT, no treatment; TSS, transcription start site; UTR, untranslated region. Data are represented as means ± sem.