Figure 4.

TAF9 is required for PU.1 gene transcription and TFIID recruitment. A, B) TAF9 was stably knocked down in K562 cells by using inducible lentiviral shRNA. TAF9 gene expression was measured by using real-time RT-PCR (A), and cell extracts were subjected to Western blot against TAF9 and β-actin Ab (B). C) PU.1 gene expression was measured by using real-time RT-PCR from cells with or without induction of TAF9 knockdown for 3 d with doxycycline (Dox). D) ChIP assay was performed in K562 cells with induced TAF9 knockdown with Ab as indicated. The resulting precipitated DNA was analyzed by using real-time PCR. CTL, control; TSS, transcription start site; UTR, untranslated region; WT, wild type. Data are represented as means ± sem.