Figure 6.

Acetylated TAF9 does not bind to chromatin and results in the disassociation of the entire TFIID complex from chromatin. A) Control and knockdown cells (shTAF9) were induced with or without doxycycline (Dox) for 3 d and transiently transfected with shTAF9-resistant TAF9 or TAF9 K5Q mutant (TAF9r or K5Qr). Cell extracts of treated cells were subjected to Western blot with indicated Abs. B, C) Knockdown cells (shTAF9) were induced with or without Dox for 3 d and transiently transfected with TAF9 or TAF9 K5Q mutant. Cells were subjected to ChIP assay with TAF9 and TAF6 (B); and TAF1, TAF5, and TBP (C). The resulting precipitated DNA was analyzed by using real-time PCR. CTL, control; TSS, transcription start site; UTR, untranslated region. Data are represented as means ± sem.