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. 2017 Aug 25;7:9457. doi: 10.1038/s41598-017-10072-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Label-free detection of bacteria in blood using protein microarrays and SPRi detection. Bacteria from an isolated bacterial colony were grown overnight in blood culture medium. Then, the culture was ten-fold serially diluted in the same medium and a diluted aliquot was finally mixed with blood samples so that the blood-to-broth ratio was kept constant (1:5) similarly to conditions routinely used in clinical settings. Several dozens of antibodies can be arrayed on 1 cm². Live bacteria are captured on microarrayed specific antibodies (spotted in triplicate onto the biochip surface) during the enrichment step. SPRi data are treated and plotted as variations of light reflectivity (ΔR (%)) over time for each region-of-interest (corresponding to antibody spots arrayed on the sensor). Differential SPR images (obtained by subtracting a reference SPR image recorded at t(0) to any SPR image acquired later in the experiment) may also be displayed.