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. 2017 Aug 25;7:9457. doi: 10.1038/s41598-017-10072-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Label-free detection of Salmonella Enteritidis contaminating a series of different samples. The SPR imaging of biochip is recorded in pure culture media; in culture media with human serum and in culture media with human blood. (A) Pure culture medium spiked with Salmonella Enteritidis (9.6 × 10³ ± 1 × 10³ CFU.mL⁻¹ at t = 0). (B) Salmonella Enteritidis (1.3 × 10⁴ ± 3.3 × 10³ CFU.mL⁻¹) detection in blood culture media containing human serum (10:1 ratio). (C) Salmonella Enteritidis (124 ± 16 CFU.mL⁻¹) detection in culture media containing human blood (10:1 ratio). (D) SPRi data for uncontaminated human blood diluted in culture medium and processed on the antibody microarray. Each curve corresponds to the averaged SPR responses recorded for a triplicate series of each antibody. (E) Arraying map and differential SPR image of the biochip incubated in 10% human serum condition (B), taken 8 hours after the experiment beginning. (F) Arraying map and differential SPR image of the biochip incubated in culture media containing 10% of human blood (C) after 8 hours.