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. 2017 Aug 25;7:9457. doi: 10.1038/s41598-017-10072-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SPRi data analysis and assessment of characteristic detection times. The detection of Salmonella Enteritidis, with an initial bacterial load of 14 ± 6 CFU.mL⁻¹, is carried in a 1 mL volume composed of blood culture medium and human blood (10:1 ratio). (A) Raw SPRi data. (B) SPRi data smoothed with a Savitzky-Golay filter. (C) First derivatives of SPRi data. (D) Second derivatives of SPRi data. Yellow curves represent the averaged SPRi signal of IgY NI and IgG controls plus or minus five standard deviations. These data thus define the threshold values for positivity assessment.