Figure 5.

Salmonella Enteritidis SPRi detection in large volumes similar to clinical routine analysis (5.4 mL of blood diluted in 26.6 mL of culture medium, for a 1:5 blood-to-broth ratio and a 32 mL total volume). (A) Photograph and scheme of the whole set-up containing the sample container, the syringe for reactor filling and the biochip. The instrument and samples were placed in an incubator at 37 °C. (B–D) SPRi data recording during the detection of 0.7 ± 0.02 CFU of Salmonella Enteritidis per milliliter of blood diluted in blood culture media with a 1:5 ratio (5.4 mL of blood diluted in 26.6 mL of culture medium) in 32 mL total volume. (B) Raw SPRi data for pathogen specific antibodies (IgG anti-Salmonella) and negative controls (IgG control, IgY NI control and IgG anti-PBP2a). Each IgG has been replicated on the microarray, and every single IgG spot response was independently followed. (C) Second SPRi derivatives for every feature arrayed on the biochip. (D) Second SPRi derivatives plotted between 300 and 600 minutes. Yellow curves represent the average signal of the control plus or minus five standard deviations.