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. 2017 Aug 25;7:9433. doi: 10.1038/s41598-017-09648-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of H₂S on SM-induced changes in Nrf2 expression, nuclear translocation. Mice were subcutaneously injected with SM (30 mg/kg), with or without intraperitoneal administration of NaHS (5 mg/kg) or intraperitoneal administration of PPG (30 mg/kg), addition NaHS and PPG were given every day for a total of three doses. The left lower lung was removed for Nrf2 protein expression examination using immunohistochemistry (A,B). Lung tissues were collected for isolation of cytosolic and nuclear proteins. Total Nrf2 (C), Nrf2 cytoplasmic (D) and nuclear (E) protein levels in lung tissues were determined by western blot analysis. Data are presented as the mean ± SEM (n = 4). *p < 0.05 vs CTRL group; **p < 0.01 vs CTRL group; ^#p < 0.05 vs SM group; ^##p < 0.01 vs SM group.