Fig. 2.

Kinetics of the heat-induced aggregation, fusion and rupture of LDL(+) and LDL(−). All data were recorded from LDL samples under standard conditions in a temperature jump from 25 °C to 82 °C. (A) The time course of the transition was monitored at 320 nm by turbidity for increase in the particle size. (B) Simultaneously, near-UV CD at 320 nm signal was recorded to monitor lipid re-packing upon LDL rupture and coalescence into lipid droplets. For LDL(+), the signal loss due to sample precipitation was observed after ∼60 min. (C) NGGE of LDL subfractions at a baseline and after 90 min of thermal denaturation. (D) Repetitive kinetic data recorded from LDL(+). The sample was subjected to a T-jump from 25 °C to 82 °C (light-grey). An identical sample was subjected to similar T-jump for 15 min, followed by rapid sample cooling on ice, equilibration at 25 °C, and the second consecutive T-jump to 82 °C (grey, *).