Table 1.

Apparent K_D values of recombinant anti-GD2 antibodies. Apparent K_D values were calculated using glycan microarray analysis.

Antibody	KD,App (nM)¶	Literature† KD (nM)	Fold Preference‡ GD2 vs. GT2	Fold Preference‡ GD2 vs. GQ2
3F8	8.5	13 ± 3	4000	250
Germline 3F8	146	--	> 5000	1000
Humanized 3F8	7.7	11 ± 1	1500	200
3F8_mut (HC:I56S)	128	--	5000	500
Dinutuximab (ch14.18)	60	77 ± 8	> 5000	1000
Germline 14.18	1600	--	> 5000	250

^¶All tested antibodies were of human IgG1 isotype.

^†Literature values, for comparative purposes, from Cheung et al. (2012)

^‡Fold preferences indicate relative binding of GD2 over GT2 or GQ2 as estimated from the array data.