Table 1. Results of in-house and commercially available Zika virus immunoassays*.

Sample collection dpo	In-house Zika virus diagnostic results			DENV PRNT titer	Commercial Zika virus serologic assays results
	RT-PCR	MAC-ELISA	PRNT titer		Euroimmun IgM	Euroimmun IgG	Novatec IgM	Abcam IgM	InBios IgM
12	ND	Pos	>40	Neg	Pos	Pos	Pos	Pos	Pos
9	ND	Pos	>40	Neg	Neg	Neg	Neg	Neg	Pos
4	ND	Pos	>40	Neg	Neg	Neg	Neg	Pos	Pos
27	ND	Pos	>40	Neg	Pos	Pos	Pos	Pos	Pos
39	ND	Pos	>40	Neg	Neg	Pos	Pos	Pos	Pos
11	ND	Pos	>40	Neg	Pos	Pos	Pos	Pos	Pos
109	ND	Pos	1,280	20	Neg	Pos	Neg	Neg	Pos
49	ND	Pos	>40	Neg	Neg	Pos	Pos	Pos	Pos
Unknown	ND	Pos	>40	Neg	Neg	Neg	Neg	Neg	Pos
4	ND	Pos	>40	Neg	Pos	Neg	Pos	Pos	Pos
7	ND	Pos	>40	Neg	Neg	Pos	Neg	Neg	Pos
46	ND	Pos	>40	Neg	Neg	Pos	Pos	Pos	Pos
Unknown	ND	Pos	>40	Neg	Neg	Pos	Neg	Neg	Pos
Unknown	ND	Pos	>40	Neg	Neg	Pos	Eq	Pos	Pos
118	ND	Pos	>40	Neg	Neg	Pos	Neg	Neg	Pos
57	ND	Pos	>40	Neg	Pos	Neg	Pos	Pos	Pos
66	ND	Pos	>40	Neg	Neg	Pos	Neg	Eq	Pos
43	ND	Pos	40	Neg	Neg	Pos	Eq	Pos	Pos
2	ND	Pos	>40	Neg	Neg	Neg	Pos	Neg	Pos
41	ND	Pos	>40	Neg	Pos	Pos	Neg	Neg	Pos
5	ND	Pos	>40	Neg	Neg	Neg	Neg	Neg	Pos
38	ND	Pos	>40	Neg	Neg	Pos	Neg	Neg	PZ
4	ND	Pos	>80	Neg	Pos	Neg	Neg	Pos	Pos
6	ND	Pos	>80	Neg	Pos	Pos	Neg	Neg	Pos
2	ND	Pos	>80	Neg	Neg	Pos	Neg	Eq	Pos
12	ND	Pos	40	Neg	Pos	Neg	Pos	Pos	Pos
Unknown	ND	Pos	>80	Neg	Pos	Neg	Pos	Pos	Pos
28	ND	Pos	>80	Neg	Pos	Pos	Neg	Neg	Pos
75	ND	Pos	>80	20	Neg	Pos	Neg	Neg	Pos
68	ND	Pos	>40	Neg	Neg	Pos	Neg	Neg	Pos
9	ND	Pos	Neg	>40	Neg	Neg	Neg	Neg	PZ
7	ND	Pos	Neg	>40	Neg	Neg	Neg	Neg	PZ
31	ND	Pos	Neg	>40	Neg	Neg	Neg	Neg	PZ
6	ND	Pos	Neg	>40	Pos	Pos	Neg	Neg	Pos
20	ND	Pos	Neg	>40	Neg	Neg	Pos	Pos	PZ
36	ND	Pos	Neg	>80	Neg	Neg	Neg	Neg	OF
Unknown	ND	Pos	320	>5,120	Neg	Neg	Neg	Neg	Pos
Unknown	ND	Pos	Neg	40	Neg	Neg	Pos	Neg	Neg
3	ND	Pos	Neg	>80	Neg	Neg	Eq	Neg	Pos
Unknown	ND	Pos	Neg	>640	Neg	Neg	Neg	Neg	Pos

*Bold indicates false-positive/false-negative results. Underlining indicates inconclusive results that required further testing. DENV, dengue virus; dpo, days postsymptom onset; Eq, equivalent; MAC-ELISA, IgM-capture ELISA; ND, not done; Neg, negative; OF, other flavivirus; Pos, positive; PRNT, plaque reduction neutralization test; PZ, possible Zika virus; RT-PCR, reverse transcription PCR.