Table 2. Transmission of cattle, porcine, sheep, and goat BSE isolates to mice in transgenic mouse lines TgMet129, TgMet/Val129, and TgVal129*.
| Isolates |
Mean survival time, d ±SD (no. PrPres-positive/inoculated animals) [reference]†‡ | |||||||
|---|---|---|---|---|---|---|---|---|
| TgMet129 |
TgMet/Val129 |
TgVal129 |
||||||
| First passage |
Second passage |
First passage |
Second passage |
First passage |
Second passage |
|||
| Hu-sCJD MM1 | 219 ±17 (6/6) [40] | 239 ±8 (6/6) [40] | 243 ±14 (6/6) [40] | 260 ±13 (6/6) [40] | 327 ±19 (6/6) [40] | 286 ±16 (6/6) [40] | ||
| Hu-sCJD VV2 | 618 ±81 (6/6) [40] | 509 ±41 (6/6) [40] | 588 ±74 (6/6) [40] | 594 ±86 (6/6) [40] | 168 ±12 (6/6) [40] | 169 ±12 (6/6) [40] | ||
| Hu-TSE negative | >700‡ (0/6) | >700‡ (0/6) | >700‡ (0/6) | >700‡ (0/6) | >700‡ (0/6) | >700‡ (0/6) | ||
| Ca-BSE0 | 739 (1/6) (31) | 633 ±32 (6/6) | >700‡ (0/6) | >700‡ (0/6) | >700‡ (0/6) | >700‡ (0/6) | ||
| Ca-BSE2 | 491–707 (0/9) [31] | 572 ±37 (3/4) [31] | >700‡ (0/5) | ND | >700‡ (0/3)§ | >700‡ (0/3)§ | ||
| Ca-BSE/TgPo | 653 ±45 (3/5) | ND | ND | ND | >700‡ (0/6) | ND | ||
| Ca-BSE/Sh(ARQ) | 615 ±84 (4/6) [31] | 564 ±39 (5/5) [31] | >700b (0/6) | >700‡ (0/6) | >700‡ (0/6) | >700‡ (0/6) | ||
| Ca-BSE/Go | >700‡ (5/5) | >700‡ (5/5) | 476 (1/10) | ND | >700‡ (0/5)¶ | ND | ||
| Go-BSE | 683 ± 36 (6/6) | 675 ± 36 (5/5) | >700‡ (0/6) | ND | >700‡ (0/6) | ND | ||
*BSE, bovine spongiform encephalopathy; Ca, cattle; CJD, Creutzfeldt-Jakob disease; Go, goat; Hu, human; Met129, methionine; ND, not done; PrPres, proteinase K–resistant PrP; sCJD, sporadic CJD; Val129, valine; vCJD, variant CJD. †Survival time is indicated for all mice that scored positive for PrPres ‡Animals without clinical signs were euthanized at 700 dpi. §Three additional animals were culled before the end of the experiment because of intercurrent illnesses; all were negative for brain PrPres by using Western blot. ¶Positive subclinical infection tested in Bo-Tg110 mice.