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. 2017 Jul 14;292(34):14050–14065. doi: 10.1074/jbc.M117.799304

Figure 5.

Figure 5.

IBTKα associates with SEC proteins. A and B, HepG2 cells were treated for 12 h with the indicated stress compounds, and cell lysates were used for immunoprecipitation with IgG control (mock), IBTKα, or SEC16A followed by immunoblot analysis. On the left of A, input indicates the immunoblot analyses of total lysates. C, shCTRL and shSEC16A HepG2 cells were treated for 12 h with vehicle or palmitate, and cell lysates were used for immunoprecipitation with IgG control (mock) or IBTKα followed by immunoblot analysis. On the left of C, input indicates the immunoblot analyses of total lysates. D and E, HepG2 cells were treated with palmitate or vehicle for 12 h, and cell lysates were used for immunoprecipitation with IgG control (mock), IBTKα, or SEC16A, followed by immunoblot analysis to measure the indicated proteins. On the left of D, input indicates immunoblot analyses carried out using total lysates. F, shCTRL and shSEC31A HepG2 cells were treated with vehicle or palmitate for 12 h, and cell lysates were used for immunoprecipitation with IgG control (mock) or IBTKα, followed by immunoblot analyses to measure the indicated proteins.