Figure 6.

Kinetics of BpPRF-catalyzed sulfur transfer reactions. The sulfurtransferase activity associated with the rhodanese domain in wild-type BpPRF (A, C, and E) or with the isolated rhodanese domain (Rhod domain) (B, D, and F) was determined in the presence of a constant thiosulfate concentration (60 mm) and varying concentrations of cyanide (A and B), GSH (C and D), or cysteine (E and F). The data are representative of 3–4 independent experiments. The data were fitted with the Michaelis-Menten, sigmoidal, or Hill equation as described under “Experimental procedures.”