Figure 7.

Kinetics of BpPRF-catalyzed sulfur transfer reactions. Dependence of sulfurtransferase activity on varying thiosulfate concentrations in the presence of 60 mm cyanide (A and B), 30 mm GSH (C and D), and 50 mm cysteine (E and F) for the wild-type BpPRF and the isolated rhodanese domain (Rhod Domain). The reactions were performed as described under “Experimental procedures.” The data are representative of 3–4 independent experiments. Data were fitted with either Michaelis-Menten or Hill equation.