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Comparison of the PDO activities of wild-type and C314S BpPRF
The kinetic parameters were determined by monitoring O2 consumption in the presence of GSSH at 22 °C and pH 7.4, as described under “Experimental procedures.”
| Enzyme | Iron content | Vmax | Km (GSSH) | Km (O2) | kcat | kcat/ Km (GSSH) | kcat/Km (O2) |
|---|---|---|---|---|---|---|---|
| mol of iron/mol | μmol min−1 mg−1 | mm | mm | s−1 | mm−1 s−1 | mm−1 s−1 | |
| Wild type | 0.60 ± 0.03 | 207 ± 6 | 0.070 ± 0.008 | 0.13 ± 0.03 | 143 | 2043 | 1100 |
| C314S | 0.46 ± 0.02 | 7.3 ± 0.1 | 0.37 ± 0.04 | 5 | 14 |
