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Affinity of Hsp70 for select RNA substrates
| RNA substrate | Kd appa | n |
|---|---|---|
| nm | ||
| Fl-ARE[44] | 7.8 ± 0.7 | 4 |
| Fl-ARE[38] | 10.9 ± 1.8 | 8 |
| Fl-ARE[34] | 12.5 ± 1.0 | 3 |
| Fl-ARE[30] | 12.6 ± 0.6 | 3 |
| Fl-ARE[24] | 380 ± 40 | 3 |
| Fl-ARE[20] | >500 | 2 |
a Apparent equilibrium dissociation constants were resolved using fluorescence anisotropy-based assays as described in Fig. 1 and are listed as the mean ± S.D. from n independent experiments.
