Table 4.
Effect of specific Hsp70 protein domains on VEGFA mRNA turnover in HeLa cells
| siRNA | ectopic Hsp70 protein | t½a | n | P vs. siHsp70 aloneb |
|---|---|---|---|---|
| h | ||||
| siControl | None | 3.78 ± 0.35 | 5 | < 0.0001 |
| siHsp70 | None | 1.84 ± 0.32 | 5 | |
| siHsp70 | FLAG-Hsp70wt | 3.86 ± 0.55 | 3 | 0.0005 |
| siHsp70 | FLAG-Hsp70(1–613) | 3.70 ± 0.29 | 3 | 0.0002 |
| siHsp70 | FLAG-Hsp70(1–385) | 1.87 ± 0.14 | 4 | NS |
| siHsp70 | FLAG-Hsp70(386–613) | 3.00 ± 0.23 | 4 | 0.0005 |
a Turnover kinetics of VEGFA mRNA were measured in HeLa cells transfected with indicated siRNAs and FLAG-Hsp70 expression vectors using actD time course assays as described under “Experimental Procedures” and in Fig. 6. Listed mRNA half-life values represent the mean ± S.D. from n independent time course experiments.
b NS, no significant difference between samples (p > 0.05).