Figure 1.
The analysis of the conformation and stability of tRNALeu(UUR). A, cloverleaf structure of human mitochondrial tRNALeu(UUR). Broken lines, anticipated tertiary base pairings, including G13-U22 and U22-G46. B, schematic model for the tertiary structure of tRNAGlu (Protein Data Bank code 2DER). Broken square, D-stem and variable loop region of tRNA. C, Northern blot analysis of tRNA under native conditions. Two μg of total mitochondrial RNA from various cell lines were electrophoresed through native polyacrylamide gel, electroblotted, and hybridized with DIG-labeled oligonucleotide probes specific for tRNALeu(UUR), tRNALys, tRNAMet, and tRNAIle, respectively. D, thermal stability of wild-type and mutant tRNALeu(UUR). ΔTm indicates the difference of Tm value between wild-type and mutant tRNALeu(UUR). The calculations were based on three independent experiments.