Figure 7.

Respiration assays. A, analysis of O₂ consumption in the various cell lines using different inhibitors. The OCRs were first measured on 2 × 10⁴ cells of each cell line under basal conditions and then with sequential additions of oligomycin (1.5 m), FCCP (0.5 m), rotenone (1.0 m), and antimycin A (1.0 m) at the indicated times to determine different parameters of mitochondrial functions. B, graphs present the ATP-linked OCR, proton leak OCR, maximal OCR, reserve capacity, and non-mitochondrial OCR in mutant and control cell lines. Non-mitochondrial OCR was determined as the OCR after rotenone/antimycin A treatment. Basal OCR was determined as OCR before oligomycin minus OCR after rotenone/antimycin A. ATP-lined OCR was determined as OCR before oligomycin minus OCR after oligomycin. Proton leak was determined as basal OCR minus ATP-linked OCR. Maximal was determined as the OCR after FCCP minus non-mitochondrial OCR. Reserve capacity was defined as the difference between maximal OCR after FCCP minus basal OCR. The average values of four independent experiments for each cell line are shown; the horizontal dashed lines represent the average value for each group. Graph details and symbols are explained in the legend to Fig. 3.