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. 2017 Jun 27;292(34):14280–14289. doi: 10.1074/jbc.M117.797100

Figure 1.

Figure 1.

Rho*-catalyzed nucleotide exchange activity of αT* in various detergents. A, tryptophan fluorescence emission profiles of Rho*-catalyzed nucleotide exchange in maltoside detergents at a concentration of 2× CMC (OM, octyl maltoside; NM, nonyl maltoside; DM, decyl maltoside; UM, undecyl maltoside; DDM, dodecyl maltoside). Inset, rate constants obtained from single exponential fits of the data (n = 3). The respective rate constants are as follows: OM, 0.96 ± 0.13 min−1; NM, 2.81 ± 0.21 min−1; DM, 3.83 ± 0.01 min−1; UM, 2.60 ± 0.67 min−1; DDM, 1.52 ± 0.17 min−1. B, tryptophan fluorescence emission profiles of Rho*-catalyzed nucleotide exchange in maltose neopentyl glycol detergents at a concentration of 2× CMC (OMNG, octyl maltose neopentyl glycol; DMNG, decyl maltose neopentyl glycol; LMNG, lauryl maltose neopentyl glycol). Inset, rate constants obtained from single exponential fits of the data (n = 3). The respective rate constants as follows: OMNG, 1.83 ± 0.52 min−1; DMNG, 3.12 ± 0.56 min−1; LMNG, 2.64 ± 0.68 min−1. C, tryptophan fluorescence emission profiles of Rho*-catalyzed nucleotide exchange in DDM at various detergent concentrations (CMC = 0.0087% w/v). D, tryptophan fluorescence emission profiles of Rho*-catalyzed nucleotide exchange in LMNG at various detergent concentrations (CMC = 0.001% w/v).