Figure 1.

Novel reporter system to detect activators of Nrf2 translation. A, schematic representation of the composition of the recombinant transcript used to identify activators of Nrf2 translation. B, expression of luciferase in HEK293T cells was evaluated by Western blotting with or without the fusion of the 3′-region of Nrf2 as depicted in A (n = 3). The relative intensity of the bands is represented as the ratio with β-actin as a loading control. The statistical significance of the difference in the levels of expression of Luc2 versus Luc2-sg3 is indicated by **, p < 0.01. C, real-time PCR (n = 3) of the transcripts of luciferase or Luciferase-Nrf2 overexpressed in HEK293T cells.