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. 2017 Jun 30;292(34):13970–13985. doi: 10.1074/jbc.M117.778134

Figure 4.

Figure 4.

Assessment of specific interactions of c-Rel at −1018 and −57 SNPs of CHGA promoter haplotype 2. A, schematic representation of the mutated constructs generated for single and double mutations at −1018- and −57-bp positions. B, differential activities of CHGA promoter haplotype SDM constructs. CHGA promoter haplotype SDM constructs were transfected into IMR-32 cells along with β-galactosidase expression plasmid. Results are expressed as mean ± S.E. of triplicate values of Gaussia luciferase/β-gal. **, p < 0.01 and ***, p < 0.001 as compared with Hap1-GLuc construct; ##, p < 0.01 as compared with Hap1−1018T-GLuc construct. C, effect of overexpression of c-Rel on activity of CHGA promoter haplotype SDM constructs. IMR-32 cells were transfected with CHGA promoter haplotype SDM constructs and increasing doses of c-Rel expression plasmid. pCDNA 3.1(+) was used as balancing plasmid. Results are expressed as mean ± S.E. of triplicates of Gaussia luciferase/μg of protein values. *, p < 0.05 and **, p < 0.01 as compared with basal activity of the corresponding CHGA promoter construct.