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. 2017 Jun 30;292(34):13970–13985. doi: 10.1074/jbc.M117.778134

Figure 5.

Figure 5.

Interactions of c-Rel with CHGA promoter −1018 and −57 SNPs in vitro: EMSA for −1018 (A), −57 (B), and c-Rel consensus (C) oligos. A and B, lanes 1 and 9, only labeled variant (Var) and wild-type (WT) oligos; lanes 2 and 10, IMR-32 nuclear extract with Var and WT biotin-labeled oligos; lanes 3-5 and 11–13, labeled oligos incubated with molar excesses of corresponding unlabeled oligos and nuclear extract; lanes 6 and 14, Var and WT-labeled oligos incubated with a mouse monoclonal c-Rel antibody and nuclear extract; lanes 7 and 15, Var and WT-labeled oligos incubated with control IgG and nuclear extract; lane 8, only IMR-32 nuclear extract. C, lane 1, only labeled c-Rel consensus oligo; lane 2, IMR-32 nuclear extract with labeled c-Rel consensus oligo; lanes 3–5, labeled c-Rel consensus oligo incubated with molar excesses of unlabeled oligo; lane 6, labeled c-Rel consensus oligo incubated with a mouse monoclonal c-Rel antibody and nuclear extract; lane 7, labeled c-Rel consensus oligo incubated with control IgG and nuclear extract; lane 8, only IMR-32 nuclear extract. Specific complexes are indicated.