Figure 7.

Kindlin-2 interacts with K2 C-terminal peptide. Lysates of αIIbβ3-CHO cells transfected with PSGL-1 alone, PSGL-1–K2CT, PSGL-1–K2CTΔ679, or PSGL-1–K2CT double mutant were used for co-IP assays. After incubating with A/G-agarose and PSGL-1 antibody, full-length K2 bound to PSGL-1 constructs was evaluated by SDS-PAGE and Western blotting using anti-K2 (Cell Signaling), which does not detect the C-terminal end of K2. PSGL-1 and K2 levels in total lysates (TL) are also shown, with actin used as a loading control.