Figure 3.

High speed egg extracts can form circular DNA. (A) Sperm nuclei were incubated in a high speed egg extract for 90 min. DNA was purified and analyzed by 2D gel electrophoresis and hybridization with a satellite 1 probe. (B and C) Charomid DNA (100 ng) was incubated in 50 µl of a high speed egg extract supplemented with 25 µCi [α-³²P]dCTP for 90 min, in the absence (B) or presence (C) of aphidicolin (50 µg/ml). DNA was purified and analyzed by 2D gel electrophoresis, blotted and directly exposed to film.