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. 2017 Aug 21;10:1973–1989. doi: 10.2147/JPR.S132682

Figure 4.

Figure 4

Alterations in protein expression of ECS components in knee joints associated with the progression of MIA-induced OA rat model.

Notes: Protein expression of CB1 and CB2 receptors (A, B), NAPE-PLD, FAAH enzymes (C, D), 15LOX2, COX2 enzymes (E, F) levels were determined by immunoblotting. Samples were collected before OA induction and 2, 7, 14, 21 and 28 days after MIA injection. The results are presented as fold of change (mean ± SEM) in comparison to the intact group (n=5). Representative bands from each group are presented below the charts. Statistical analysis was performed using one-way analysis of variance followed by Bonferroni post hoc test; values with p<0.05 were considered significant. *Denotes significant differences vs int (before OA induction) at p<0.05, **denotes significant differences vs int (before OA induction) at p<0.01, ***denotes significant differences vs int (before OA induction) at p<0.005, ****denotes significant differences vs int (before OA induction) at p<0.001, #denotes significant differences vs day 2 post-MIA injection at p<0.05, ##denotes significant differences vs day 2 post-MIA injection at p<0.01, ###denotes significant differences vs day 2 post-MIA injection at p<0.001, $denotes significant differences vs indicated bar at p<0.05, $$denotes significant differences vs indicated bar at p<0.01.

Abbreviations: ECS, endocannabinoid system; MIA, monoiodoacetate; OA, osteoarthritis; FAAH, fatty acid amide hydrolase; NAPE-PLD, N-acyl phosphatidylethanolamine phospholipase D; SEM, standard error of the mean; int, intact animals.