Table 3.
Summary of gene expression alterations revealed in rat knee model during progression of OA
| Gene description | Time after MIA injection
|
|||
|---|---|---|---|---|
| Day 7 | Day 14 | Day 21 | Day 28 | |
| Progression markers | ||||
| Sox9 | ↑↑↑↑ | ↑↑ | ↑↑ | |
| Comp | ↑↑↑↑ | ↑↑ | ↑↑↑↑ | ↑↑↑ |
| MMPs/TIMPs | ||||
| Mmp3 | – | ↑↑↑↑ | ↑↑↑↑ | – |
| Mmp9 | – | ↑↑ | – | – |
| Mmp13 | – | – | ↑ | ↑↑ |
| Timp1 | ↑ | – | ↑ | ↑ |
| Timp2 | ↑↑↑↑ | ↑ | ↑↑ | ↑↑↑↑ |
| ECS receptors | ||||
| Cb1 | – | – | – | – |
| Cb2 | – | – | – | – |
| EC synthesis | ||||
| Napepld | – | – | – | – |
| Pla2g2a | – | – | – | – |
| Plcb1 | – | – | – | ↑ |
| Gde1 | – | – | – | ↑↑↑↑ |
| EC degradation | ||||
| Faah | – | – | – | |
| Ptgs2 | – | – | – | ↑↑ |
| Alox 12 | – | – | – | |
| Alox 15 | ↓ | ↓ | – | ↓ |
Notes: Transcripts abundance levels of selected genes in joint tissue samples were assessed by quantitative polymerase chain reaction. Total RNA samples were collected 2, 7, 14, 21 and 28 days after OA induction. The results are presented as relative increase (↑) or decrease (↓) of mRNA levels in comparison to day 2 (n=8–10). Data were analyzed with one-way analysis of variance followed by Bonferroni’s multiple comparison test. (↑/↓) denotes significant differences vs. day 2 post MIA injection, (↑) for p<0.05, (↑↑) for p<0.01, (↑↑↑) for p<0.005, (↑↑↑↑) for p<0.001.
Abbreviations: EC, endocannabinoid; ECS, endocannabinoid system; MIA, monoiodoacetate; MMP, matrix metalloproteinase; OA, osteoarthritis; TIMP, tissue inhibitors of metalloproteinase.