Table S1.

Mean CT values for all genes under investigation

Gene description	Gene symbol	Time after MIA injection
		Day2	Day 7	Day 14	Day 21	Day 28
Reference gene	Hprt1	25.13±0,12	25.38±0.17	25.78±0.21	25.78±0.17	26.04±0.14
Progression markers	Sox9	32.56±0.37	29.86±0.23	32.53±0.55	31.41±0.42	31.16±0.27
	Comp	25.65±0.41	21.67±0.26	23.16±0.41	22.95±0.44	22,72±0.23
MMPs/TIMPs	Mmp3	24.27±0.30	23.57±0.27	23.47±0.38	23.37±0.27	24.03±0.15
	Mmp9	27.27±0,55	25.74±0.25	25.64±0.58	25.50±0.38	25.57±0.19
	Mmp13	26.36±0.46	25.95±0.37	24.60±0.54	24.28±0.36	24.46±0.18
	Timp1	24.23±0.28	23.81±0.57	24.57±0.50	24.04±0.35	24.16±0.16
	Timp2	24.94±0.18	23.29±0.23	24.34±0.34	24.14±0.29	24.09±0.17
ECS receptors	Cb1	32.56±0.18	32.46±0.28	33.11±0.33	32.90±0.17	33.35±0.20
	Cb2	32.03±0.30	32.54±0.43	33.66±0.54	33.28±0.31	33.82±0.20
EC synthesis	Napepld	30.55±0.20	30.62±0.40	31.23±0.36	31.19±0.27	31.21±0.13
	Pla2g2a	27.66±0.27	28.71±0.39	28.14±0.45	28.61±0.32	29.02±0.19
	Plcb1	28.53±0.25	28.26±0.47	28.51±0.37	28.54±0.43	28.40±0.24
	Gde1	25.64±0.19	25.87±0.50	26.06±0.38	25.71±0.26	25.50±0.17
EC degradation	Faah	33.62±0.32	33.20±0.31	34.19±0.41	34.01±0.32	34.25±0.25
	Ptgs2	30.02±0.32	28.93±0.53	30.21±0.49	29.77±0.36	29.11±0.22
	Alox 12	31.23±0.59	31.48±0.52	31.70±0.76	31.20±0.46	32.21±0.21
	Alox 15	28.61±0.62	30.13±0.58	30.38±0.74	30.50±0.56	31.10±0.28

Notes: Transcripts abundance levels of selected genes in joint tissue samples were assessed by quantitative polymerase chain reaction. Total RNA samples were collected 2, 7, 14, 21 and 28 days after osteoarthritis induction. The CT values are presented as a mean and SEM (n = 8–10).

Abbreviations: CT, cycle treshold; EC, endocannabinoid; ECS, endocannabinoid system; MMP, matrix metalloproteinase; SEM, standard error of the mean; TIMP, tissue inhibitors of metalloproteinase.