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Deletion of rpoS attenuates the inhibitory effect of prestarvation. Activation of the phoA-yfp reporter in plasmid pRG161 was assayed in the WT strain and rpoS deletion strain RU1646 (rpoS−) with or without prestarvation. Gray-shaded areas indicate growth under the Pi-replete condition. Total fluorescence (A) and the increased fluorescence for the WT (B) and RU1646 (C) strains are shown as averages of results from 10 individual wells from one microplate assay.
