TABLE 3.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| E. coli strains | ||
| CC118λpir | Δ(ara-leu)araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir | 60 |
| S17-1λpir | Tpr Smr recA thi pro rK− mK+ RP4::2-Tc::MuKm Tn7 λpir | 61 |
| V. cholerae strains | ||
| FY_VC_0001 | O1 El Tor A1552, wild type, Rifr | 62 |
| FY_VC_0237 | FY_VC_0001 mTn7 gfp Rifr Gmr | 46 |
| FY_VC_2272 | ΔVC0665 (vpsR) | 24 |
| FY_VC_9332 | ΔVCA0565 (vxrA) | 33 |
| FY_VC_8758 | ΔVCA0566 (vxrB) | 33 |
| FY_VC_0099 | ΔVCA0952 (vpsT) | 27 |
| FY_VC_9369 | ΔVCA0567 (vxrC) | 33 |
| FY_VC_9417 | ΔVCA0568 (vxrD) | 33 |
| FY_VC_9394 | ΔVCA0569 (vxrE) | 33 |
| FY_VC_9469 | ΔvxrB Tn7::vxrB | 33 |
| FY_VC_9952 | ΔvxrB Δhcp1 Δhcp2 | 33 |
| FY_VC_9569 | ΔVC1415 ΔVCA0017 (Δhcp1 Δhcp2) | 33 |
| FY_VC_9390 | ΔvxrA mTn7 gfp Rifr Gmr | This study |
| FY_VC_8764 | ΔvxrB mTn7 gfp Rifr Gmr | This study |
| FY_VC_9392 | ΔvxrC mTn7 gfp Rifr Gmr | This study |
| FY_VC_9437 | ΔvxrD mTn7 gfp Rifr Gmr | This study |
| FY_VC_9439 | ΔvxrE mTn7 gfp Rifr Gmr | This study |
| FY_VC_9234 | ΔvpsR ΔvxrB Rifr | This study |
| FY_VC_9237 | ΔvpsT ΔvxrB Rifr | This study |
| FY_VC_11356 | FY_VC_0001 mTn7 PTAC-vpsR Rifr Gmr | This study |
| FY_VC_11357 | ΔvxrA mTn7 PTAC-vpsR Rifr Gmr | This study |
| FY_VC_11358 | ΔvxrB mTn7 PTAC-vpsR Rifr Gmr | This study |
| FY_VC_11355 | ΔvxrB ΔvxrC | This study |
| FY_VC_12056 | ΔvxrA-Tn7::vxrA | This study |
| FY_VC_12057 | ΔvxrC-Tn7::vxrC | This study |
| Plasmids | ||
| pGP704sacB28 | pGP704 derivative, mob/oriT sacB Apr | 27 |
| pUX-BF13 | oriR6K helper plasmid, mob/oriT, provides the Tn7 transposition function in trans, Apr | 63 |
| pMCM11 | pGP704::mTn7 gfp Gmr Apr | M. Miller and G. Schoolnik |
| pBBRlux | luxCDABE-based promoter fusion vector, Cmr | 64 |
| pFY-0950 | pBBRlux vpsL promoter, Cmr | 65 |
| pFY-0989 | pBBRlux vpsR promoter, Cmr | This study |
| pFY-0988 | pBBRlux vpsT promoter, Cmr | This study |
| pBAD/myc | Arabinose-inducible expression vector with C-terminal myc epitope and six-His tags | |
| His-B | ||
| pFY-2074 | pBAD-vxrB-noTag Ampr | This study |
| pFY-3071 | pBAD-vxrBD78A-noTag Ampr | This study |
| pFY-3073 | pBAD-vxrBD78E-noTag Ampr | This study |