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Spectroscopic and biochemical characterization of the MBP-EB1 protein. (A) Absorbance spectra of the dark and lit protein states. (B) Kinetics of dark recovery of EB1 from lit to dark states. Plotted are changes in the A494 over time following excitation with blue light. (C) Kinetics of c-di-GMP hydrolysis by EB1 in lit and dark states. The PDE activity assay was performed by using 5 μM MBP-EB1 and initiated by the addition of c-di-GMP.
