TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| E. coli strains | ||
| MG1655 yhjH | MG1655 yhjH::Kmr | 24 |
| BL21(DE3) | Strain for protein overproduction and curli fimbria synthesis; chromosomally integrated T7 polymerase gene | NEB |
| DH5α | Strain for cloning and MBP fusion overexpression | NEB |
| Plasmids | ||
| pMal-c5x | Vector for overexpression of MBP fusion proteins | NEB |
| pMal_BldP1 | MBP::(PAS9-GGDEF-EAL-BLUF) from BldP1 | This study |
| pMal_EB1 | MBP::(EAL-BLUF) from BldP1 (plus α-helix; aa 706–1115) | This study |
| pBAD/Myc-HisB | pBAD series empty vector; Para | Invitrogen |
| pBAD-EB1 | pBAD::(EAL-BLUF) from BldP1 (plus α-helix; aa 706–1115) | This study |
| pBAD_EB1-L | pBAD::(EAL-BLUF) from BldP1 (minus α-helix; aa 716–1115) | This study |
| pBAD_EB1_E712P | pBAD::(EAL-BLUF) from BldP1 (plus α-helix; E712P mutation; aa 706–1115) | This study |
| pMal_BldP2 | MBP::(PAS9-GGDEF-EAL-BLUF) from BldP2 | This study |
| pMal_PAS-GGDEF | MBP::(PAS9-GGDEF) from BldP2 | This study |
| pMal_EB2 | MBP::(EAL-BLUF) from BldP2 (aa 694–1124) | This study |
| pBAD_EB2 | pBAD encoding the EAL-BLUF module (plus α-helix; aa 694–1124) of BldP2 | This study |
| pMslr1143 | MBP::Slr1143 | 17 |
| pET23a | Protein overexpression vector; PT7; Apr | Novagen |
| pbSHE | pET23a::(bphS-bphO-eb1) | This study |
| pbSHY2 | pET23a::(bphS-bphO-yhjH; RBS2) | 13 |
| pbSHY3 | pET23a::(bphS-bphO-yhjH; RBS3) | 13 |
| pbSHY4 | pET23a::(bphS-bphO-yhjH; RBS4) | 13 |