TABLE 2.
Primers used in this study
| Primer name | Sequence | Usea |
|---|---|---|
| algRintF | GCAACTGGACTGGCAGGTGC | Mutant |
| algRintR | CGCGACTGGTCATCGGCAG | Mutant |
| algRBamHIR | GCGCGGATCCGTCAGAGCTGATGCATCAGACG | Mutant |
| algZHindIIIF | GCGCAAGCTTCTCTCGCTGCAACAAGAAACGG | Mutant |
| algZSDMcheckF | CAGCTGGGCGGAGAACTGAC | Mutant |
| algRD54XbaIF | AACATCCGCATGCCCGGTCTAGACGGC | Mutant |
| algRD54NR | CAGCAGGACGATATCGGGCTTGA | Mutant |
| algRD54EF | GTCCTTCTAGAGATCCGCATGC | Mutant |
| algRD54ER | GATATCGGGCTTGAGGCTGTCG | Mutant |
| algZHSDMF | GAATTCCTGTTCAACAGCCTGAACAG | Mutant |
| algZHSDMR | CGGGCGAATCCGCGCCTGCA | Mutant |
| algRSalIF | CCTGTCGACATGAATGTCCTGATTGTCGATGAC | AlgR purification |
| algRNotIR | AGCCGCGGCCGCTCAGAGCTGATGCATCAGACG | AlgR purification |
| rsmARXbaI | GCGCTCTAGAGCACGGTGATCCTGCAGACC | Mutant |
| rsmAFHindIII | GCGCAAGCTTCGGCAACATCACCACGCTGGG | Mutant |
| rsmASDMcheckF | GCCAAGGTTTCCATCGTCGG | PCR check |
| rsmYRPAF | CTTGGACGTCAGGACATTGCGCAGGAAG | Northern blot |
| rsmYT7RPAR | TAATACGACTCACTATAGGGAGACAATAAAAAACCCCGCCTTTTGGG | Northern blot |
| rsmZRPAF | GGGCCCCACTCCTGCGTACAGGGAACAC | Northern blot |
| rsmZRPART7 | TAATACGACTCACTATAGGGAGACAAAAAAAAGGGGCGGGGTATTAC | Northern blot |
| rsmYTFFEcoRI | GCGCGAATTCGTGTTGCCGTCGGTCCGGC | TF |
| rsmYTFRBamHI | GCGCGGATCCTTCCTGCGCAATGTCCTGACG | TF |
| rsmYTFFUAS | GCGCGCGGCCGCCAGCGTGTAAGCCAAGGCTTAC | TF |
| rsmYshortEcoRIF | GCGCGAATTCAAACGGCGGTGGTTTTGGCTG | TF |
| dnrTFRBamHI | GCGCGGATCCCCATGCTGGGAAGGCTCGC | TF |
| rsmZKpnIF | GCGCGGTACCCGTCTGGCGCAGAAGGGCG | Mutant |
| rsmZXbaIR | GCGCTCTAGAGAGCGAAACCGCCAACATCC | Mutant |
| rsmZSOEF | GTTTTCTGGCAGGTTGCGGGATTTGCCTGCCGTTTTACTCGTC | Mutant |
| rsmZSOER | GACGAGTAAAACGGCAGGCAAATCCCGCAACCTGCCAGAAAAC | Mutant |
| rsmZintF | GCGCGCGGCCGCACCGATCCGTGCGAGCTG | Mutant |
| rsmZintR | CATGCAGGAATTCATCGAGCTG | Mutant |
| rsmYKOF | CGCGGAGCTCCTGTTCACTCGAAGCACTCCAG | Mutant |
| rsmYKOR | CGCGTCTAGATTCGCCAACTCCGCTATTTCG | Mutant |
| rsmYSOEF | GCGTAATCTTCAAACCGTCAGGATCCTGCGGCCCGAGGAAAACCGCGTCG | Mutant |
| rsmYSOER | CGACGCGGTTTTCCTCGGGCCGCAGGATCCTGACGGTTTGAAGATTACGC | Mutant |
| rsmYintF | CCTGGAGCTGGACGGGAG | Mutant |
| rsmYintR | GGAATTCAGGAAGGTGTCCC | Mutant |
| lacUV5NotI | GGCCGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGAG | Leader fusion vector |
| lacUV5BamHI | GATCCTCCACACATTATACGAGCCGGAAGCATAAACTGTAAAGC | Leader fusion vector |
| tssA1annealF | GATCCTTCGATGATAGGGAGATCGTCACCGTGCTG | Leader fusion |
| tssA1annealR | CAGCACGGTGACGATCTCCCTATCATCGAAG | Leader fusion |
| hcnAannealF | GATCCACTCTCTCTCACGGATGAAAGGGCAATGCAC | Leader fusion |
| hcnAannealR | GTGCATTGCCCTTTCATCCGTGAGAGAGAGTG | Leader fusion |
| 5SrRNAI | TTGGACAGGATGGGGTTGGA | Northern blot |
| 5SrRNA2T7 | TAATACGACTCACTATAGGGA GACGATTGTGTGTTGTAAGG | Northern blot |
| lacZScaIF | GCTATGACCATGATTAGTACTGATTCACTGGCCGTC | TLF vector |
| lacZRXhoI | CCCCTCGAGCAGACATGGCCTGCCCGGTTATTA | TLF vector |
| lacZRforTF | GATGTGCTGCAAGGCGATTAAG | SEQ |
| rsmASDMAlgRF | GAATTCGGCAGGAACTTTCATTCCGGC | RPA |
| rsmARPAR | TAATACGACTCACTATAGGGAGAGATAAAAATTAATGGTTTGGCTCTTG | RPA |
| rsmARSDMF | CCTTTTGTCGTTTTTGAGAATTCGGCAGGAACTTTCATTC | Gel shift |
| rsmARSDMR | GAATGAAAGTTCCTGCCGAATTCTCAAAAACGACAAAAGG | Gel shift |
| algDBS1F | CGCCTCCTGGCGCTACCGTTCGTCCCTCCGACACCCCTGCTGCGTCGC | Gel shift |
| algDBS1R | GCGACGCAGCAGGGGTGTCGGAGGGACGAACGGTAGCGCCAGGAGGCG | Gel shift |
| rsmARBS1F | GCGCCTTTTGTCGTTTTTGACCGTTTGGCAGGAACTTTCATTCCG | Gel shift |
| rsmARBS1R | CGGAATGAAAGTTCCTGCCAAACGGTCAAAAACGACAAAAGGCGC | Gel shift |
| pscFRBSF | GCTGGCGGAGTGTCGCCGGGAACTGGCCAGAGG | Gel shift |
| pscFRBSR | CCTCTGGCCAGTTCCCGGCGACACTCCGCCAGC | Gel shift |
| rsmAPE1F | GCGCGGTACCAAGGATCGCGCTCTTGATTTCTGCGGATCCGCCGCCATTTCTT | Gel shift |
| rsmAPE3F | GCGCGGTACCGCTGACAGGCGAAAGGCG | Gel shift |
| rsmAPE3R | GCGCGGATCCTCACCCAGTATTGACCAGTCC | Gel shift |
| rsmAEcoRIR | GCGCGAATTCACGAGTCAGAATCAGCATTCCTTTC | Gel shift |
| rsmAR1SDMannealF | CCGCGGAATTCGTCGTTTTTGACCGTTTGGCAGGAACTTTC | Gel shift |
| rsmAR1SDMannealR | GAAAGTTCCTGCCAAACGGTCAAAAACGACGAATTCCGCGG | Gel shift |
| rsmAR2SDMannealF | CCGCGGAATTCGTCGTTTTTGAGAATTCGGCAGGAACTTTC | Gel shift |
| rsmAR2SDMannealR | GAAAGTTCCTGCCGAATTCTCAAAAACGACGAATTCCGCGG | Gel shift |
| rsmAGSWTannealF | CCGCGCCTTTTGTCGTTTTTGACCGTTTGGCAGGAACTTTC | Gel shift |
| rsmAGSWTannealR | GAAAGTTCCTGCCAAACGGTCAAAAACGACAAAAGGCGCGG | Gel shift |
a
TF, transcriptional fusion; TLF, translational fusion; SEQ, sequencing primer; RPA, RNase protection assay.