FIG 2.

Effects of gluR-K deletion on bacterial growth and antibiotic biosynthesis of S. coelicolor. (A) Phenotypic analysis of the ΔgluR-K mutant grown on MM with 75 mM glutamate. Three S. coelicolor strains, including M145/pSET152, the ΔgluR-K mutant with pSET152 (ΔgluR-K/pSET152), and the complemented ΔgluR-K/pSET-gluR-K strain, were cultured for two different time intervals (48 and 96 h) before imaging. (B and C) Growth curves (B) and quantitative analysis of ACT production (C). Three S. coelicolor strains were grown on MM with 75 mM glutamate covered with sterile plastic cellophane. The samples were collected at five time points as indicated.